![]() We will now convert the test into a smaller, faster, cheaper version of the test that examines only the relevant RNA transcripts that we have identified. We have completed the first 112 patients using internal philanthropic funds and very generous collaborative support from our colleagues. ![]() In the future, this test could be expanded toward diagnosing CAD in asymptomatic patients, which could potentially prevent unexpected MI and provide physicians the chance for early intervention, with simple, proven therapies such as aspirin, statins, and lifestyle changes. These studies potentially provide a clinic-ready diagnostic test for the presence of CAD in chest pain patients. Careful analysis and confirmatory studies strongly suggest that these TRACs are RNA markers of subset of T cells, consistent with numerous prior publications suggesting changes in the T cell ratios in CAD. Further, the low fold changes may explain why these transcripts have not been seen in prior microarray-based studies, although 2-3 transcripts do agree with prior published results. The resulting short reads were aligned to the human transcriptome and the number of reads per kilobase of exon per million (RPKM) reads was determined, and then compared between groups by filtering for changes >1.5 fold at a p value 1.5-fold, but less than 2-fold, which could be consistent with changes in the numbers of a specific circulating cell type. Blood RNA was depleted of ribosomal RNA and then RNA was sequenced on a SeqLL SMS. A development cohort of 112 patients presenting for coronary catheterization was subjected to RNAseq. Whole blood RNA was analyzed by the most advanced SeqLL single molecule sequencing (SMS) of RNA (RNAseq, aka ‘deep or next-gen sequencing’) to identify transcripts associated with CAD (TRACs). Patients who develop coronary artery disease produce specific changes in gene expression in whole blood RNA that are not present in patients without coronary artery disease. There is an urgent need for a blood test that will predict the presence of CAD in patients presenting with a clinical suspicion of heart disease. return a result of ‘no coronary blockage’. Surprisingly, despite some well-established clinical and diagnostic criteria, about 30-40% of the 1 million diagnostic catheterizations each year in the U.S. The current gold standard for diagnosing coronary artery disease (CAD) is coronary artery angiography. Atherosclerosis can lead to an acute myocardial infarction (MI), or heart attack, with an incidence of approximately 650,000 per year in the U.S. Laurent InstituteĬardiovascular diseases, particularly atherosclerosis, are the major cause of death and morbidity in developed countries. Laurent, III, Phillip Kapranov, & Dmitry Shtokalo SeqLL, Inc. Tim McCaffrey, Ian Toma, Richard Katz, Jon Reiner, & Ramesh Mazhari Divisions of Genomic Medicine and Cardiology The George Washington University Medical Center In Partnership with Georges St. “Our researchers study microarray, gene transfer, and DNA sequencing to extend the capabilities of our clinicians as they formulate treatment plans and guide patient care.” The Development of a Blood Test for Coronary Artery Disease ![]() Here is our first Case study listed below, and please make sure to check back in the future for more case studies & updates. It secures the device properly while traveling or commuting.Ĭonvenient design includes a smooth but strong double zipper which is easy for opening and closing, giving you a better user experience.Įco-friendly Material made of medium density foam.We have recently added a Case Study Section to our website so you can see how tSMS technology is being utilized by leading researchers for multiple cutting edge applications. It prevents any damages or scratches caused by accidental bumping or dropping. The case is semi-water resistant, anti-static, shockproof, stylish and durable. If you are a long time qwerty keyboard user with concerns about learning an entirely new way to type, you may want to consider CharaChorder Lite as an alternative.Īll CharaChorder One orders include a complimentary travel case! CharaChorder One features the first ever layout designed for both character entry and chorded entry, as well as 3-D switches rather than traditional 1-D keyboard switches. The CharaChorder One is an entirely new class of peripheral device which unabashedly casts aside all the rules of traditional typewriting and challenges the status quo of human computer interaction.
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